2N9H

Glucose as a nuclease mimic in DNA

Summary for 2N9H

• Entry DOI: 10.2210/pdb2n9h/pdb
• Related: 2N9F
• NMR Information: BMRB: 25906
• Descriptor: DNA (5'-D(*GP*AP*TP*GP*AP*CP*TP*GP*CP*TP*AP*G)-3'), DNA (5'-D(*CP*TP*AP*GP*CP*(GL6)P*GP*TP*CP*AP*TP*C)-3') (2 entities in total)
• Functional Keywords: carbohydrate-dna interaction, glucose-nucleobase, dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 2
• Total formula weight: 7311.80

Authors

Gomez-Pinto, I.,Vengut-Climent, E.,Lucas, R.,Avino, A.,Eritja, R.,Gonzalez-Ibanez, C.,Morales, J.,Muro, A.,Penalver, P.,Fonseca-Guerra, C.,Bickelhaupt, M. (deposition date: 2015-11-25, release date: 2016-08-31, Last modification date: 2024-05-15)

Primary citation

Vengut-Climent, E.,Gomez-Pinto, I.,Lucas, R.,Penalver, P.,Avino, A.,Fonseca Guerra, C.,Bickelhaupt, F.M.,Eritja, R.,Gonzalez, C.,Morales, J.C.
Glucose-Nucleobase Pseudo Base Pairs: Biomolecular Interactions within DNA.
Angew.Chem.Int.Ed.Engl., 55:8643-8647, 2016

PubMed Abstract: Noncovalent forces rule the interactions between biomolecules. Inspired by a biomolecular interaction found in aminoglycoside-RNA recognition, glucose-nucleobase pairs have been examined. Deoxyoligonucleotides with a 6-deoxyglucose insertion are able to hybridize with their complementary strand, thus exhibiting a preference for purine nucleobases. Although the resulting double helices are less stable than natural ones, they present only minor local distortions. 6-Deoxyglucose stays fully integrated in the double helix and its OH groups form two hydrogen bonds with the opposing guanine. This 6-deoxyglucose-guanine pair closely resembles a purine-pyrimidine geometry. Quantum chemical calculations indicate that glucose-purine pairs are as stable as a natural T-A pair.

PubMed: 27328804
DOI: 10.1002/anie.201603510

Experimental method

SOLUTION NMR