2N9F

Glucose as non natural nucleobase

2N9F の概要

• エントリーDOI: 10.2210/pdb2n9f/pdb
• 関連するPDBエントリー: 2N9H
• NMR情報: BMRB: 25903
• 分子名称: DNA (5'-D(*CP*TP*AP*GP*CP*GP*GP*TP*CP*AP*TP*C)-3'), DNA (5'-D(*GP*AP*TP*GP*AP*CP*(4JA)P*GP*CP*TP*AP*G)-3') (2 entities in total)
• 機能のキーワード: carbohydrate-dna interaction, carbohydrate nucleobase, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7336.81

構造登録者

Gomez-Pinto, I.,Vengut-Climent, E.,Lucas, R.,Avino, A.,Eritja, R.,Gonzalez-Ibanez, C.,Morales, J.,Penalver, P.,Fonseca-Guerra, C.,Bickelhaupt, M. (登録日: 2015-11-20, 公開日: 2016-08-31, 最終更新日: 2024-05-15)

主引用文献

Vengut-Climent, E.,Gomez-Pinto, I.,Lucas, R.,Penalver, P.,Avino, A.,Fonseca Guerra, C.,Bickelhaupt, F.M.,Eritja, R.,Gonzalez, C.,Morales, J.C.
Glucose-Nucleobase Pseudo Base Pairs: Biomolecular Interactions within DNA.
Angew.Chem.Int.Ed.Engl., 55:8643-8647, 2016

PubMed Abstract: Noncovalent forces rule the interactions between biomolecules. Inspired by a biomolecular interaction found in aminoglycoside-RNA recognition, glucose-nucleobase pairs have been examined. Deoxyoligonucleotides with a 6-deoxyglucose insertion are able to hybridize with their complementary strand, thus exhibiting a preference for purine nucleobases. Although the resulting double helices are less stable than natural ones, they present only minor local distortions. 6-Deoxyglucose stays fully integrated in the double helix and its OH groups form two hydrogen bonds with the opposing guanine. This 6-deoxyglucose-guanine pair closely resembles a purine-pyrimidine geometry. Quantum chemical calculations indicate that glucose-purine pairs are as stable as a natural T-A pair.

PubMed: 27328804
DOI: 10.1002/anie.201603510

実験手法

SOLUTION NMR