2N9C
NRAS Isoform 5
Summary for 2N9C
| Entry DOI | 10.2210/pdb2n9c/pdb |
| NMR Information | BMRB: 25900 |
| Descriptor | GTPase NRas (1 entity in total) |
| Functional Keywords | nras, signaling protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Cell membrane ; Lipid-anchor ; Cytoplasmic side : P01111 |
| Total number of polymer chains | 1 |
| Total formula weight | 2120.49 |
| Authors | Markowitz, J.,Mal, T.K.,Yuan, C.,Courtney, N.B.,Patel, M.,Stiff, A.R.,Blachly, J.,Walker, C.,Eisfeld, A.,de la Chapelle, A.,Carson III, W.E. (deposition date: 2015-11-13, release date: 2016-03-23, Last modification date: 2024-05-15) |
| Primary citation | Markowitz, J.,Mal, T.K.,Yuan, C.,Courtney, N.B.,Patel, M.,Stiff, A.R.,Blachly, J.,Walker, C.,Eisfeld, A.K.,de la Chapelle, A.,Carson, W.E. Structural characterization of NRAS isoform 5. Protein Sci., 25:1069-1074, 2016 Cited by PubMed Abstract: It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix-turn-coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy. PubMed: 26947772DOI: 10.1002/pro.2916 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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