2N9C

NRAS Isoform 5

2N9C の概要

• エントリーDOI: 10.2210/pdb2n9c/pdb
• NMR情報: BMRB: 25900
• 分子名称: GTPase NRas (1 entity in total)
• 機能のキーワード: nras, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane ; Lipid-anchor ; Cytoplasmic side : P01111
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2120.49

構造登録者

Markowitz, J.,Mal, T.K.,Yuan, C.,Courtney, N.B.,Patel, M.,Stiff, A.R.,Blachly, J.,Walker, C.,Eisfeld, A.,de la Chapelle, A.,Carson III, W.E. (登録日: 2015-11-13, 公開日: 2016-03-23, 最終更新日: 2024-05-15)

主引用文献

Markowitz, J.,Mal, T.K.,Yuan, C.,Courtney, N.B.,Patel, M.,Stiff, A.R.,Blachly, J.,Walker, C.,Eisfeld, A.K.,de la Chapelle, A.,Carson, W.E.
Structural characterization of NRAS isoform 5.
Protein Sci., 25:1069-1074, 2016

PubMed Abstract: It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix-turn-coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy.

PubMed: 26947772
DOI: 10.1002/pro.2916

実験手法

SOLUTION NMR