2N9B

Solution NMR Structure of Antiparallel Myosin-10:GCN4 Tandem Coiled-Coil

2N9B の概要

• エントリーDOI: 10.2210/pdb2n9b/pdb
• 関連するPDBエントリー: 2lw9 2ZTA
• NMR情報: BMRB: 25899
• 分子名称: Unconventional myosin-X, General control protein GCN4 fusion (1 entity in total)
• 機能のキーワード: anti-parallel coiled-coil, coiled-coil, motor protein-transcription complex, motor protein/transcription
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16400.58

構造登録者

Vavra, K.C.,Xia, Y.,Rock, R.S. (登録日: 2015-11-12, 公開日: 2016-06-15, 最終更新日: 2024-05-15)

主引用文献

Vavra, K.C.,Xia, Y.,Rock, R.S.
Competition between Coiled-Coil Structures and the Impact on Myosin-10 Bundle Selection
Biophys.J., 110:2517-2527, 2016

PubMed Abstract: Coiled-coil fusions are a useful approach to enforce dimerization in protein engineering. However, the final structures of coiled-coil fusion proteins have received relatively little attention. Here, we determine the structural outcome of adjacent parallel and antiparallel coiled coils. The targets are coiled coils that stabilize myosin-10 in single-molecule biophysical studies. We reveal the solution structure of a short, antiparallel, myosin-10 coiled-coil fused to the parallel GCN4-p1 coiled coil. Surprisingly, this structure is a continuous, antiparallel coiled coil where GCN4-p1 pairs with myosin-10 rather than itself. We also show that longer myosin-10 segments in these parallel/antiparallel fusions are dynamic and do not fold cooperatively. Our data resolve conflicting results on myosin-10 selection of actin filament bundles, demonstrating the importance of understanding coiled-coil orientation and stability.

PubMed: 27276269
DOI: 10.1016/j.bpj.2016.04.048

実験手法

SOLUTION NMR