2N93

Solution structure of lcFABP

2N93 の概要

• エントリーDOI: 10.2210/pdb2n93/pdb
• NMR情報: BMRB: 25400
• 分子名称: Fatty acid-binding protein (1 entity in total)
• 機能のキーワード: lipid binding protein
• 由来する生物種: Luciola cerata
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14450.55

構造登録者

Tseng, K.,Lyu, P. (登録日: 2015-11-05, 公開日: 2016-01-20, 最終更新日: 2024-05-15)

主引用文献

Tseng, K.L.,Lee, Y.Z.,Chen, Y.R.,Lyu, P.C.
1H, 15N and 13C resonance assignments of light organ-associated fatty acid-binding protein of Taiwanese fireflies.
Biomol NMR Assign, 10:71-74, 2016

PubMed Abstract: Fatty acid-binding proteins (FABPs) are a family of proteins that modulate the transfer of various fatty acids in the cytosol and constitute a significant portion in many energy-consuming cells. The ligand binding properties and specific functions of a particular type of FABP seem to be diverse and depend on the respective binding cavity as well as the cell type from which this protein is derived. Previously, a novel FABP (lcFABP; lc: Luciola cerata) was identified in the light organ of Taiwanese fireflies. The lcFABP was proved to possess fatty acids binding capabilities, especially for fatty acids of length C14-C18. However, the structural details are unknown, and the structure-function relationship has remained to be further investigated. In this study, we finished the (1)H, (15)N and (13)C chemical shift assignments of (15)N/(13)C-enriched lcFABP by solution NMR spectroscopy. In addition, the secondary structure distribution was revealed based on the backbone N, H, Cα, Hα, C and side chain Cβ assignments. These results can provide the basis for further structural exploration of lcFABP.

PubMed: 26373428
DOI: 10.1007/s12104-015-9640-0

実験手法

SOLUTION NMR