2N8X

Solution structure of LptE from Pseudomonas Aerigunosa

2N8X の概要

• エントリーDOI: 10.2210/pdb2n8x/pdb
• 関連するPDBエントリー: 2JXP 2R76 3BF2 4N4R 4NHR 4Q35
• NMR情報: BMRB: 25869
• 分子名称: LPS-assembly lipoprotein LptE (1 entity in total)
• 機能のキーワード: lps biosynthesis, lipid binding protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• 細胞内の位置: Cell outer membrane ; Lipid-anchor : Q9HX32
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18411.46

構造登録者

Moehle, K.,Kocherla, H.,Jurt, S.,Robinson, J.,Zerbe, O.,Zerbe, K.,Bacsa, B. (登録日: 2015-10-28, 公開日: 2016-05-25, 最終更新日: 2024-05-01)

主引用文献

Moehle, K.,Kocherla, H.,Bacsa, B.,Jurt, S.,Zerbe, K.,Robinson, J.A.,Zerbe, O.
Solution Structure and Dynamics of LptE from Pseudomonas aeruginosa.
Biochemistry, 55:2936-2943, 2016

PubMed Abstract: LptE is an outer membrane (OM) lipoprotein found in Gram-negative bacteria, where it forms a complex with the β-barrel lipopolysaccharide (LPS) transporter LptD. The LptD/E complex plays a key role in OM biogenesis, by translocating newly synthesized LPS molecules from the periplasm into the external leaflet of the asymmetric OM during cell growth. The LptD/E complex in Pseudomonas aeruginosa (Pa) is a target for macrocyclic β-hairpin-shaped peptidomimetic antibiotics, which inhibit the transport of LPS to the cell surface. So far, the three-dimensional structure of the Pa LptD/E complex and the mode of interaction with these antibiotics are unknown. Here, we report the solution structure of a Pa LptE derivative lacking the N-terminal lipid membrane anchor, determined by multidimensional solution nuclear magnetic resonance (NMR) spectroscopy. The structure reveals a central five-stranded β-sheet against which pack a long C-terminal and a short N-terminal α-helix, as found in homologues of LptE from other Gram-negative bacteria. One unique feature is an extended C-terminal helix in Pa LptE, which in a model of the Pa LptD/E complex appears to be long enough to contact the periplasmic domain of LptD. Chemical shift mapping experiments suggest only weak interactions occur between LptE and the oligosaccharide chains of LPS. The NMR structure of Pa LptE will be valuable for more detailed structural studies of the LptD/E complex from P. aeruginosa.

PubMed: 27166502
DOI: 10.1021/acs.biochem.6b00313

実験手法

SOLUTION NMR