2N8O

NMR Solution Structure of Aureocin A53

2N8O の概要

• エントリーDOI: 10.2210/pdb2n8o/pdb
• 関連するPDBエントリー: 2N8P
• NMR情報: BMRB: 25857
• 分子名称: Bacteriocin aureocin A53 (1 entity in total)
• 機能のキーワード: bacteriocin, leaderless bacteriocin, antimicrobial protein
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Secreted: Q8GPI4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5994.23

構造登録者

Acedo, J.Z.,van Belkum, M.J.,Lohans, C.T.,Towle, K.M.,Miskolzie, M.,Vederas, J.C. (登録日: 2015-10-22, 公開日: 2016-02-17, 最終更新日: 2024-05-01)

主引用文献

Acedo, J.Z.,van Belkum, M.J.,Lohans, C.T.,Towle, K.M.,Miskolzie, M.,Vederas, J.C.
Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.
Biochemistry, 55:733-742, 2016

PubMed Abstract: Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four α-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 Å) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against the Gram-positive strains tested, suggesting that sequence disparities play a crucial role in their mechanisms of action.

PubMed: 26771761
DOI: 10.1021/acs.biochem.5b01306

実験手法

SOLUTION NMR