2N82

solution structure of the complex of microRNA 20b pre-element with Rbfox RRM

2N82 の概要

• エントリーDOI: 10.2210/pdb2n82/pdb
• 関連するPDBエントリー: 2N7X
• NMR情報: BMRB: 25831
• 分子名称: RNA (5'-R(*GP*GP*UP*AP*GP*UP*UP*UP*UP*GP*GP*CP*AP*UP*GP*AP*CP*UP*CP*UP*AP*CP*C)-3'), RNA binding protein fox-1 homolog 1 (2 entities in total)
• 機能のキーワード: microrna, rrm, rbfox, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q9NWB1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19149.67

構造登録者

Yang, F.,Chen, Y.,Varani, G. (登録日: 2015-09-30, 公開日: 2016-04-13, 最終更新日: 2024-05-15)

主引用文献

Chen, Y.,Zubovic, L.,Yang, F.,Godin, K.,Pavelitz, T.,Castellanos, J.,Macchi, P.,Varani, G.
Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer.
Nucleic Acids Res., 44:4381-4395, 2016

PubMed Abstract: Rbfox proteins regulate tissue-specific splicing by targeting a conserved GCAUG sequence within pre-mRNAs. We report here that sequence-specific binding of the conserved Rbfox RRM to miRNA precursors containing the same sequence motif in their terminal loops, including miR-20b and miR-107, suppresses their nuclear processing. The structure of the complex between precursor miR-20b and Rbfox RRM shows the molecular basis for recognition, and reveals changes in the stem-loop upon protein binding. In mammalian cells, Rbfox2 downregulates mature miR-20b and miR-107 levels and increases the expression of their downstream targets PTEN and Dicer, respectively, suggesting that Rbfox2 indirectly regulates many more cellular miRNAs. Thus, some of the widespread cellular functions of Rbfox2 protein are attributable to regulation of miRNA biogenesis, and might include the mis-regulation of miR-20b and miR-107 in cancer and neurodegeneration.

PubMed: 27001519
DOI: 10.1093/nar/gkw177

実験手法

SOLUTION NMR