2N7S

Solution Structure of Leptospiral LigA4 Big Domain

「2N2I」から置き換えられました

2N7S の概要

• エントリーDOI: 10.2210/pdb2n7s/pdb
• NMR情報: BMRB: 25819
• 分子名称: Ig-like repeat domain protein 1 (1 entity in total)
• 機能のキーワード: pdz, structural protein
• 由来する生物種: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9481.26

構造登録者

Mei, S. (登録日: 2015-09-17, 公開日: 2015-10-21, 最終更新日: 2024-05-15)

主引用文献

Mei, S.,Zhang, J.,Zhang, X.,Tu, X.
Solution structure of leptospiral LigA4 Big domain.
Biochem. Biophys. Res. Commun., 467:288-292, 2015

PubMed Abstract: Pathogenic Leptospiraspecies express immunoglobulin-like proteins which serve as adhesins to bind to the extracellular matrices of host cells. Leptospiral immunoglobulin-like protein A (LigA), a surface exposed protein containing tandem repeats of bacterial immunoglobulin-like (Big) domains, has been proved to be involved in the interaction of pathogenic Leptospira with mammalian host. In this study, the solution structure of the fourth Big domain of LigA (LigA4 Big domain) from Leptospira interrogans was solved by nuclear magnetic resonance (NMR). The structure of LigA4 Big domain displays a similar bacterial immunoglobulin-like fold compared with other Big domains, implying some common structural aspects of Big domain family. On the other hand, it displays some structural characteristics significantly different from classic Ig-like domain. Furthermore, Stains-all assay and NMR chemical shift perturbation revealed the Ca(2+) binding property of LigA4 Big domain.

PubMed: 26449456
DOI: 10.1016/j.bbrc.2015.09.170

実験手法

SOLUTION NMR