2N74

Solution Structure of the RNA-Binding domain of non-structural protein 1 from the 1918 H1N1 influenza virus

2N74 の概要

• エントリーDOI: 10.2210/pdb2n74/pdb
• NMR情報: BMRB: 25793
• 分子名称: Non-structural protein 1 (1 entity in total)
• 機能のキーワード: ns1, rig-i, virulence, viral protein
• 由来する生物種: Influenza A virus
• 細胞内の位置: Host nucleus: Q99AU3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16818.99

構造登録者

Jureka, A.S.,Kleinpeter, A.B.,Cornilescu, G.,Cornilescu, C.C.,Schwieters, C.D.,Petit, C.M. (登録日: 2015-09-03, 公開日: 2015-09-30, 最終更新日: 2024-05-15)

主引用文献

Jureka, A.S.,Kleinpeter, A.B.,Cornilescu, G.,Cornilescu, C.C.,Petit, C.M.
Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I.
Structure, 23:2001-2010, 2015

PubMed Abstract: The influenza non-structural protein 1 (NS1) plays a critical role in antagonizing the innate immune response to infection. One interaction that facilitates this function is between NS1 and RIG-I, one of the main sensors of influenza virus infection. While NS1 and RIG-I are known to interact, it is currently unclear whether this interaction is direct or if it is mediated by other biomolecules. Here we demonstrate a direct, strain-dependent interaction between the NS1 RNA binding domain (NS1(RBD)) of the influenza A/Brevig Mission/1918 H1N1 (1918(H1N1)) virus and the second caspase activation and recruitment domain of RIG-I. Solving the solution structure of the 1918(H1N1) NS1(RBD) revealed features in a functionally novel region that may facilitate the observed interaction. The biophysical and structural data herein suggest a possible mechanism by which strain-specific differences in NS1 modulate influenza virulence.

PubMed: 26365801
DOI: 10.1016/j.str.2015.08.007

実験手法

SOLUTION NMR