2N73

Solution structure of the ACBD3:PI4KB complex

2N73 の概要

• エントリーDOI: 10.2210/pdb2n73/pdb
• 関連するPDBエントリー: 2N72
• NMR情報: BMRB: 25791
• 分子名称: Golgi resident protein GCP60, Phosphatidylinositol 4-kinase beta (2 entities in total)
• 機能のキーワード: kinase, transferase-transferase regulator complex, transferase/transferase regulator
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side : Q9H3P7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16303.34

構造登録者

Veverka, V.,Hexnerova, R. (登録日: 2015-09-02, 公開日: 2016-07-20, 最終更新日: 2024-05-15)

主引用文献

Klima, M.,Toth, D.J.,Hexnerova, R.,Baumlova, A.,Chalupska, D.,Tykvart, J.,Rezabkova, L.,Sengupta, N.,Man, P.,Dubankova, A.,Humpolickova, J.,Nencka, R.,Veverka, V.,Balla, T.,Boura, E.
Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein.
Sci Rep, 6:23641-23641, 2016

PubMed Abstract: Phosphatidylinositol 4-kinase beta (PI4KB) is one of four human PI4K enzymes that generate phosphatidylinositol 4-phosphate (PI4P), a minor but essential regulatory lipid found in all eukaryotic cells. To convert their lipid substrates, PI4Ks must be recruited to the correct membrane compartment. PI4KB is critical for the maintenance of the Golgi and trans Golgi network (TGN) PI4P pools, however, the actual targeting mechanism of PI4KB to the Golgi and TGN membranes is unknown. Here, we present an NMR structure of the complex of PI4KB and its interacting partner, Golgi adaptor protein acyl-coenzyme A binding domain containing protein 3 (ACBD3). We show that ACBD3 is capable of recruiting PI4KB to membranes both in vitro and in vivo, and that membrane recruitment of PI4KB by ACBD3 increases its enzymatic activity and that the ACBD3:PI4KB complex formation is essential for proper function of the Golgi.

PubMed: 27009356
DOI: 10.1038/srep23641

実験手法

SOLUTION NMR