2N6W
NMR Assignment and structure of CssA Thermometer from Neisseria meningitidis
Summary for 2N6W
| Entry DOI | 10.2210/pdb2n6w/pdb |
| Related | 2N6S 2N6T 2N6X |
| NMR Information | BMRB: 25784 |
| Descriptor | RNA (68-MER) (1 entity in total) |
| Functional Keywords | rna regulation, pathogen, rna thermometer, rna, structural genomics, seattle structural genomics center for infectious disease, ssgcid |
| Biological source | Neisseria meningitidis |
| Total number of polymer chains | 1 |
| Total formula weight | 21654.72 |
| Authors | Barnwal, R.,Godin, K.,Varani, G.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2015-08-28, release date: 2016-08-03, Last modification date: 2024-05-01) |
| Primary citation | Barnwal, R.P.,Loh, E.,Godin, K.S.,Yip, J.,Lavender, H.,Tang, C.M.,Varani, G. Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis. Nucleic Acids Res., 44:9426-9437, 2016 Cited by PubMed Abstract: Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes in temperature. RNA thermometers within mRNAs control expression of bacterial immune evasion factors, including CssA, in the 5'-untranslated region of the operon for capsule biosynthesis. We dissect the molecular mechanisms of thermoregulation and report the structure of the CssA thermometer. We show that the RNA thermometer acts as a rheostat, whose stability is optimized to respond in a small temperature range around 37°C as occur within the upper airways during infection. Small increases in temperature gradually open up the structure to allow progressively increased access to the ribosome binding site. Even small changes in stability induced by mutations of imperfect base pairs, as in naturally occurring polymorphisms, shift the thermometer response outside of the desired temperature range, suggesting that its activity could be modulated by pharmacological intervention. PubMed: 27369378DOI: 10.1093/nar/gkw584 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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