2N6S

Structure of CssA4 (bottom stem) of CssA thermometer

2N6S の概要

• エントリーDOI: 10.2210/pdb2n6s/pdb
• 関連するPDBエントリー: 2N6T 2N6W 2N6X
• NMR情報: BMRB: 25780
• 分子名称: RNA (36-MER) (1 entity in total)
• 機能のキーワード: rna regulation, pathogen, rna thermometer, rna, structural genomics, seattle structural genomics center for infectious disease, ssgcid
• 由来する生物種: Neisseria meningitidis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11474.78

構造登録者

Barnwal, R.,Godin, K.,Varani, G.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2015-08-28, 公開日: 2016-08-03, 最終更新日: 2024-05-15)

主引用文献

Barnwal, R.P.,Loh, E.,Godin, K.S.,Yip, J.,Lavender, H.,Tang, C.M.,Varani, G.
Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis.
Nucleic Acids Res., 44:9426-9437, 2016

PubMed Abstract: Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes in temperature. RNA thermometers within mRNAs control expression of bacterial immune evasion factors, including CssA, in the 5'-untranslated region of the operon for capsule biosynthesis. We dissect the molecular mechanisms of thermoregulation and report the structure of the CssA thermometer. We show that the RNA thermometer acts as a rheostat, whose stability is optimized to respond in a small temperature range around 37°C as occur within the upper airways during infection. Small increases in temperature gradually open up the structure to allow progressively increased access to the ribosome binding site. Even small changes in stability induced by mutations of imperfect base pairs, as in naturally occurring polymorphisms, shift the thermometer response outside of the desired temperature range, suggesting that its activity could be modulated by pharmacological intervention.

PubMed: 27369378
DOI: 10.1093/nar/gkw584

実験手法

SOLUTION NMR