2N5C

Solution NMR structure of the lasso peptide chaxapeptin

2N5C の概要

• エントリーDOI: 10.2210/pdb2n5c/pdb
• NMR情報: BMRB: 25704
• 分子名称: chaxapeptin (1 entity in total)
• 機能のキーワード: chaxapeptin, ripps, genome mining, atacama desert, streptomyces, cell invasion
• 由来する生物種: Streptomyces leeuwenhoekii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1633.82

構造登録者

Elsayed, S.S.,Trusch, F.,Deng, H.,Raab, A.,Prokes, I.,Busarakam, K.,Asenjo, J.A.,Andrews, B.A.,van West, P.,Bull, A.T.,Goodfellow, M.,Yi, Y.,Ebel, R.,Jaspars, M.,Rateb, M.E. (登録日: 2015-07-14, 公開日: 2015-10-07, 最終更新日: 2024-11-20)

主引用文献

Elsayed, S.S.,Trusch, F.,Deng, H.,Raab, A.,Prokes, I.,Busarakam, K.,Asenjo, J.A.,Andrews, B.A.,van West, P.,Bull, A.T.,Goodfellow, M.,Yi, Y.,Ebel, R.,Jaspars, M.,Rateb, M.E.
Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert.
J.Org.Chem., 80:10252-10260, 2015

PubMed Abstract: Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique "lariat knot" structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549.

PubMed: 26402731
DOI: 10.1021/acs.joc.5b01878

実験手法

SOLUTION NMR