2N52

The solution structure of the kallikrein inhibitor SPINK6

2N52 の概要

• エントリーDOI: 10.2210/pdb2n52/pdb
• NMR情報: BMRB: 25691
• 分子名称: Serine protease inhibitor Kazal-type 6 (1 entity in total)
• 機能のキーワード: inhibitor, hydrolase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : Q6UWN8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6411.24

構造登録者

Grotzinger, J. (登録日: 2015-07-06, 公開日: 2016-03-16, 最終更新日: 2024-11-06)

主引用文献

Jung, S.,Fischer, J.,Spudy, B.,Kerkow, T.,Sonnichsen, F.D.,Xue, L.,Bonvin, A.M.,Goettig, P.,Magdolen, V.,Meyer-Hoffert, U.,Grotzinger, J.
The solution structure of the kallikrein-related peptidases inhibitor SPINK6.
Biochem.Biophys.Res.Commun., 471:103-108, 2016

PubMed Abstract: Kallikrein-related peptidases (KLKs) are crucial for epidermal barrier function and are involved in the proteolytic regulation of the desquamation process. Elevated KLK levels were reported in atopic dermatitis. In skin, the proteolytic activity of KLKs is regulated by specific inhibitors of the serine protease inhibitor of Kazal-type (SPINK) family. SPINK6 was shown to be expressed in human stratum corneum and is able to inhibit several KLKs such as KLK4, -5, -12, -13 and -14. In order to understand the structural traits of the specific inhibition we solved the structure of SPINK6 in solution by NMR-spectroscopy and studied its interaction with KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors.

PubMed: 26828269
DOI: 10.1016/j.bbrc.2016.01.172

実験手法

SOLUTION NMR