2N4K

Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31

Summary for 2N4K

• Entry DOI: 10.2210/pdb2n4k/pdb
• NMR Information: BMRB: 25670
• Descriptor: Enterocin-HF (1 entity in total)
• Functional Keywords: antilisterial, gram-positive, antimicrobial protein
• Biological source: Enterococcus faecium
• Cellular location: Secreted : P86183
• Total number of polymer chains: 1
• Total formula weight: 4336.91

Authors

Arbulu, S.,Lohans, C.T.,van Belkum, M.J.,Cintas, L.M.,Herranz, C.,Vederas, J.C.,Hernandez, P.E. (deposition date: 2015-06-21, release date: 2015-12-02, Last modification date: 2016-01-06)

Primary citation

Arbulu, S.,Lohans, C.T.,van Belkum, M.J.,Cintas, L.M.,Herranz, C.,Vederas, J.C.,Hernandez, P.E.
Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.
J.Agric.Food Chem., 63:10689-10695, 2015

PubMed Abstract: The solution structure of enterocin HF (EntHF), a class IIa bacteriocin of 43 amino acids produced by Enterococcus faecium M3K31, was evaluated by CD and NMR spectroscopy. Purified EntHF was unstructured in water, but CD analysis supports that EntHF adopts an α-helical conformation when exposed to increasing concentrations of trifluoroethanol. Furthermore, NMR spectroscopy indicates that this bacteriocin adopts an antiparallel β-sheet structure in the N-terminal region (residues 1-17), followed by a well-defined central α-helix (residues 19-30) and a more disordered C-terminal end (residues 31-43). EntHF could be structurally organized into three flexible regions that might act in a coordinated manner. This is in agreement with the absence of long-range nuclear Overhauser effect signals between the β-sheet domain and the C-terminal end of the bacteriocin. The 3D structure recorded for EntHF fits emerging facts regarding target recognition and mode of action of class IIa bacteriocins.

PubMed: 26585399
DOI: 10.1021/acs.jafc.5b03882

Experimental method

SOLUTION NMR