2N4K

Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31

2N4K の概要

• エントリーDOI: 10.2210/pdb2n4k/pdb
• NMR情報: BMRB: 25670
• 分子名称: Enterocin-HF (1 entity in total)
• 機能のキーワード: antilisterial, gram-positive, antimicrobial protein
• 由来する生物種: Enterococcus faecium
• 細胞内の位置: Secreted : P86183
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4336.91

構造登録者

Arbulu, S.,Lohans, C.T.,van Belkum, M.J.,Cintas, L.M.,Herranz, C.,Vederas, J.C.,Hernandez, P.E. (登録日: 2015-06-21, 公開日: 2015-12-02, 最終更新日: 2024-11-20)

主引用文献

Arbulu, S.,Lohans, C.T.,van Belkum, M.J.,Cintas, L.M.,Herranz, C.,Vederas, J.C.,Hernandez, P.E.
Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.
J.Agric.Food Chem., 63:10689-10695, 2015

PubMed Abstract: The solution structure of enterocin HF (EntHF), a class IIa bacteriocin of 43 amino acids produced by Enterococcus faecium M3K31, was evaluated by CD and NMR spectroscopy. Purified EntHF was unstructured in water, but CD analysis supports that EntHF adopts an α-helical conformation when exposed to increasing concentrations of trifluoroethanol. Furthermore, NMR spectroscopy indicates that this bacteriocin adopts an antiparallel β-sheet structure in the N-terminal region (residues 1-17), followed by a well-defined central α-helix (residues 19-30) and a more disordered C-terminal end (residues 31-43). EntHF could be structurally organized into three flexible regions that might act in a coordinated manner. This is in agreement with the absence of long-range nuclear Overhauser effect signals between the β-sheet domain and the C-terminal end of the bacteriocin. The 3D structure recorded for EntHF fits emerging facts regarding target recognition and mode of action of class IIa bacteriocins.

PubMed: 26585399
DOI: 10.1021/acs.jafc.5b03882

実験手法

SOLUTION NMR