2N4J

Solution structure of a self complementary Xylonucleic Acid duplex

2N4J の概要

• エントリーDOI: 10.2210/pdb2n4j/pdb
• NMR情報: BMRB: 25669
• 分子名称: XNA (5'-R(*(8XG)P*(8XU)P*(8XG)P*(8XU)P*(8XA)P*(8XC)P*(8XA)P*(8XC))-3') (1 entity in total)
• 機能のキーワード: unknown function
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5053.12

構造登録者

Maiti, M.,Lescrinier, E.,Herdewijn, P. (登録日: 2015-06-19, 公開日: 2015-07-29, 最終更新日: 2024-05-15)

主引用文献

Maiti, M.,Maiti, M.,Knies, C.,Dumbre, S.,Lescrinier, E.,Rosemeyer, H.,Ceulemans, A.,Herdewijn, P.
Xylonucleic acid: synthesis, structure, and orthogonal pairing properties.
Nucleic Acids Res., 43:7189-7200, 2015

PubMed Abstract: There is a common interest for studying xeno-nucleic acid systems in the fields of synthetic biology and the origin of life, in particular, those with an engineered backbone and possessing novel properties. Along this line, we have investigated xylonucleic acid (XyloNA) containing a potentially prebiotic xylose sugar (a 3'-epimer of ribose) in its backbone. Herein, we report for the first time the synthesis of four XyloNA nucleotide building blocks and the assembly of XyloNA oligonucleotides containing all the natural nucleobases. A detailed investigation of pairing and structural properties of XyloNAs in comparison to DNA/RNA has been performed by thermal UV-melting, CD, and solution state NMR spectroscopic studies. XyloNA has been shown to be an orthogonal self-pairing system which adopts a slightly right-handed extended helical geometry. Our study on one hand, provides understanding for superior structure-function (-pairing) properties of DNA/RNA over XyloNA for selection as an informational polymer in the prebiotic context, while on the other hand, finds potential of XyloNA as an orthogonal genetic system for application in synthetic biology.

PubMed: 26175047
DOI: 10.1093/nar/gkv719

実験手法

SOLUTION NMR