2N2Y

Solution structure of the meiosis-expressed gene 1 (Meig1)

2N2Y の概要

• エントリーDOI: 10.2210/pdb2n2y/pdb
• NMR情報: BMRB: 25627
• 分子名称: Meiosis-expressed gene 1 protein (1 entity in total)
• 機能のキーワード: cell cycle
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11256.78

構造登録者

Williams Jr., D.C.,Walavalkar, N.M.,Buchwald, W.A. (登録日: 2015-05-16, 公開日: 2016-01-20, 最終更新日: 2024-05-15)

主引用文献

Li, W.,Walavalkar, N.M.,Buchwald, W.A.,Teves, M.E.,Zhang, L.,Liu, H.,Bilinovich, S.,Peterson, D.L.,Strauss Iii, J.F.,Williams, D.C.,Zhang, Z.
Dissecting the structural basis of MEIG1 interaction with PACRG.
Sci Rep, 6:18278-18278, 2016

PubMed Abstract: The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail.

PubMed: 26726850
DOI: 10.1038/srep18278

実験手法

SOLUTION NMR