2N2O

Structure of murine tumour necrosis factor alpha CDE RNA

2N2O の概要

• エントリーDOI: 10.2210/pdb2n2o/pdb
• 関連するPDBエントリー: 2N2P
• NMR情報: BMRB: 25603
• 分子名称: RNA (5'-R(P*GP*CP*AP*UP*GP*UP*UP*UP*UP*CP*UP*GP*UP*GP*AP*AP*AP*AP*CP*GP*GP*UP*U)-3') (1 entity in total)
• 機能のキーワード: cde, rna, murine, wild type
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7343.35

構造登録者

Codutti, L.,Leppek, K.,Zalesak, J.,Windeisen, V.,Masiewicz, P.,Stoecklin, G.,Carlomagno, T. (登録日: 2015-05-11, 公開日: 2015-08-05, 最終更新日: 2024-05-15)

主引用文献

Codutti, L.,Leppek, K.,Zalesak, J.,Windeisen, V.,Masiewicz, P.,Stoecklin, G.,Carlomagno, T.
A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin.
Structure, 23:1437-1447, 2015

PubMed Abstract: The constitutive decay element (CDE) of tumor necrosis factor α (TNF-α) mRNA (Tnf) represents the prototype of a class of RNA motifs that mediate rapid degradation of mRNAs encoding regulators of the immune response and development. CDE-type RNAs are hairpin structures featuring a tri-nucleotide loop. The protein Roquin recognizes CDE-type stem loops and recruits the Ccr4-Caf1-Not deadenylase complex to the mRNA, thereby inducing its decay. Stem recognition does not involve nucleotide bases; however, there is a strong stem sequence requirement for functional CDEs. Here, we present the solution structures of the natural Tnf CDE and of a CDE mutant with impaired Roquin binding. We find that the two CDEs adopt unique and distinct structures in both the loop and the stem, which explains the ability of Roquin to recognize stem loops in a sequence-specific manner. Our findings result in a relaxed consensus motif for prediction of new CDE stem loops.

PubMed: 26165594
DOI: 10.1016/j.str.2015.06.001

実験手法

SOLUTION NMR