2N1Q

HIV-1 Core Packaging Signal

2N1Q の概要

• エントリーDOI: 10.2210/pdb2n1q/pdb
• NMR情報: BMRB: 25571
• 分子名称: RNA_(155-MER) (1 entity in total)
• 機能のキーワード: rna, hiv-1, packaging signal
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50544.08

構造登録者

Keane, S.C.,Summers, M.F. (登録日: 2015-04-15, 公開日: 2015-05-27, 最終更新日: 2024-05-15)

主引用文献

Keane, S.C.,Heng, X.,Lu, K.,Kharytonchyk, S.,Ramakrishnan, V.,Carter, G.,Barton, S.,Hosic, A.,Florwick, A.,Santos, J.,Bolden, N.C.,McCowin, S.,Case, D.A.,Johnson, B.A.,Salemi, M.,Telesnitsky, A.,Summers, M.F.
RNA structure. Structure of the HIV-1 RNA packaging signal.
Science, 348:917-921, 2015

PubMed Abstract: The 5' leader of the HIV-1 genome contains conserved elements that direct selective packaging of the unspliced, dimeric viral RNA into assembling particles. By using a (2)H-edited nuclear magnetic resonance (NMR) approach, we determined the structure of a 155-nucleotide region of the leader that is independently capable of directing packaging (core encapsidation signal; Ψ(CES)). The RNA adopts an unexpected tandem three-way junction structure, in which residues of the major splice donor and translation initiation sites are sequestered by long-range base pairing and guanosines essential for both packaging and high-affinity binding to the cognate Gag protein are exposed in helical junctions. The structure reveals how translation is attenuated, Gag binding promoted, and unspliced dimeric genomes selected, by the RNA conformer that directs packaging.

PubMed: 25999508
DOI: 10.1126/science.aaa9266

実験手法

SOLUTION NMR