2N17
NMR structure of a Kazal-type serine protease inhibitor from the subterranean termite defense gland of Coptotermes formosanus Shiraki soldiers
Replaces: 2M25Summary for 2N17
| Entry DOI | 10.2210/pdb2n17/pdb |
| NMR Information | BMRB: 18896 |
| Descriptor | Lysozyme-Protease Inhibitor Protein (1 entity in total) |
| Functional Keywords | kazal-type, serine protease inhibitor, chymotrypsin, elastase, termite, soldier, defense gland, secretion, hydrolase |
| Biological source | Coptotermes formosanus (Formosan subterranean termite) |
| Total number of polymer chains | 1 |
| Total formula weight | 8578.58 |
| Authors | Negulescu, H.,Guo, Y.,Garner, T.P.,Goodwin, O.Y.,Henderson, G.,Laine, R.A.,Macnaughtan, M.A. (deposition date: 2015-03-23, release date: 2015-05-06, Last modification date: 2024-11-06) |
| Primary citation | Negulescu, H.,Guo, Y.,Garner, T.P.,Goodwin, O.Y.,Henderson, G.,Laine, R.A.,Macnaughtan, M.A. A Kazal-Type Serine Protease Inhibitor from the Defense Gland Secretion of the Subterranean Termite Coptotermes formosanus Shiraki. Plos One, 10:e0125376-e0125376, 2015 Cited by PubMed Abstract: Coptotermes formosanus is an imported, subterranean termite species with the largest economic impact in the United States. The frontal glands of the soldier caste termites comprising one third of the body mass, contain a secretion expelled through a foramen in defense. The small molecule composition of the frontal gland secretion is well-characterized, but the proteins remain to be identified. Herein is reported the structure and function of one of several proteins found in the termite defense gland secretion. TFP4 is a 6.9 kDa, non-classical group 1 Kazal-type serine protease inhibitor with activity towards chymotrypsin and elastase, but not trypsin. The 3-dimensional solution structure of TFP4 was solved with nuclear magnetic resonance spectroscopy, and represents the first structure from the taxonomic family, Rhinotermitidae. Based on the structure of TFP4, the protease inhibitor active loop (Cys(8) to Cys(16)) was identified. PubMed: 25978745DOI: 10.1371/journal.pone.0125376 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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