2N17

NMR structure of a Kazal-type serine protease inhibitor from the subterranean termite defense gland of Coptotermes formosanus Shiraki soldiers

「2M25」から置き換えられました

2N17 の概要

• エントリーDOI: 10.2210/pdb2n17/pdb
• NMR情報: BMRB: 18896
• 分子名称: Lysozyme-Protease Inhibitor Protein (1 entity in total)
• 機能のキーワード: kazal-type, serine protease inhibitor, chymotrypsin, elastase, termite, soldier, defense gland, secretion, hydrolase
• 由来する生物種: Coptotermes formosanus (Formosan subterranean termite)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8578.58

構造登録者

Negulescu, H.,Guo, Y.,Garner, T.P.,Goodwin, O.Y.,Henderson, G.,Laine, R.A.,Macnaughtan, M.A. (登録日: 2015-03-23, 公開日: 2015-05-06, 最終更新日: 2024-11-06)

主引用文献

Negulescu, H.,Guo, Y.,Garner, T.P.,Goodwin, O.Y.,Henderson, G.,Laine, R.A.,Macnaughtan, M.A.
A Kazal-Type Serine Protease Inhibitor from the Defense Gland Secretion of the Subterranean Termite Coptotermes formosanus Shiraki.
Plos One, 10:e0125376-e0125376, 2015

PubMed Abstract: Coptotermes formosanus is an imported, subterranean termite species with the largest economic impact in the United States. The frontal glands of the soldier caste termites comprising one third of the body mass, contain a secretion expelled through a foramen in defense. The small molecule composition of the frontal gland secretion is well-characterized, but the proteins remain to be identified. Herein is reported the structure and function of one of several proteins found in the termite defense gland secretion. TFP4 is a 6.9 kDa, non-classical group 1 Kazal-type serine protease inhibitor with activity towards chymotrypsin and elastase, but not trypsin. The 3-dimensional solution structure of TFP4 was solved with nuclear magnetic resonance spectroscopy, and represents the first structure from the taxonomic family, Rhinotermitidae. Based on the structure of TFP4, the protease inhibitor active loop (Cys(8) to Cys(16)) was identified.

PubMed: 25978745
DOI: 10.1371/journal.pone.0125376

実験手法

SOLUTION NMR