2N13

Complex structure of MyUb (1080-1122) of human Myosin VI with K63-diUb

2N13 の概要

• エントリーDOI: 10.2210/pdb2n13/pdb
• 関連するPDBエントリー: 2N0Z 2N10 2N11 2N12
• NMR情報: BMRB: 25545
• 分子名称: Unconventional myosin-VI, Ubiquitin (3 entities in total)
• 機能のキーワード: motor protein-protein binding complex, motor protein/protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Isoform 3: Cytoplasmic vesicle, clathrin- coated vesicle membrane. Isoform 4: Cytoplasmic vesicle, clathrin- coated vesicle membrane: Q9UM54; Ubiquitin: Cytoplasm : P0CG47 P0CG47
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27653.73

構造登録者

He, F.,Walters, K. (登録日: 2015-03-20, 公開日: 2016-03-09, 最終更新日: 2024-10-30)

主引用文献

He, F.,Wollscheid, H.P.,Nowicka, U.,Biancospino, M.,Valentini, E.,Ehlinger, A.,Acconcia, F.,Magistrati, E.,Polo, S.,Walters, K.J.
Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains.
Cell Rep, 14:2683-2694, 2016

PubMed Abstract: Myosin VI is critical for cargo trafficking and sorting during early endocytosis and autophagosome maturation, and abnormalities in these processes are linked to cancers, neurodegeneration, deafness, and hypertropic cardiomyopathy. We identify a structured domain in myosin VI, myosin VI ubiquitin-binding domain (MyUb), that binds to ubiquitin chains, especially those linked via K63, K11, and K29. Herein, we solve the solution structure of MyUb and MyUb:K63-linked diubiquitin. MyUb folds as a compact helix-turn-helix-like motif and nestles between the ubiquitins of K63-linked diubiquitin, interacting with distinct surfaces of each. A nine-amino-acid extension at the C-terminal helix (Helix2) of MyUb is required for myosin VI interaction with endocytic and autophagic adaptors. Structure-guided mutations revealed that a functional MyUb is necessary for optineurin interaction. In addition, we found that an isoform-specific helix restricts MyUb binding to ubiquitin chains. This work provides fundamental insights into myosin VI interaction with ubiquitinated cargo and functional adaptors.

PubMed: 26971995
DOI: 10.1016/j.celrep.2016.01.079

実験手法

SOLUTION NMR