2N0R

RNA structure determination by solid-state NMR spectroscopy

Summary for 2N0R

• Entry DOI: 10.2210/pdb2n0r/pdb
• NMR Information: BMRB: 25534
• Descriptor: RNA (5'-R(*GP*CP*UP*GP*AP*GP*CP*UP*CP*GP*AP*AP*AP*GP*AP*GP*CP*AP*AP*UP*GP*AP*UP*GP*UP*C)-3') (1 entity in total)
• Functional Keywords: rna
• Total number of polymer chains: 1
• Total formula weight: 8407.08

Authors

Marchanka, A.,Simon, B.,Althoff-Ospelt, G.,Carlomagno, T. (deposition date: 2015-03-12, release date: 2015-05-20, Last modification date: 2024-05-15)

Primary citation

Marchanka, A.,Simon, B.,Althoff-Ospelt, G.,Carlomagno, T.
RNA structure determination by solid-state NMR spectroscopy.
Nat Commun, 6:7024-7024, 2015

PubMed Abstract: Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize—and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.

PubMed: 25960310
DOI: 10.1038/ncomms8024

Experimental method

SOLID-STATE NMR