2N0M

The solution structure of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae

2N0M の概要

• エントリーDOI: 10.2210/pdb2n0m/pdb
• NMR情報: BMRB: 18636
• 分子名称: Lipid modified azurin protein, COPPER (I) ION (2 entities in total)
• 機能のキーワード: neisseria, copper protein, azurin-family, metal binding protein, electron transfer
• 由来する生物種: Neisseria gonorrhoeae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13713.86

構造登録者

Pauleta, S.R.,Matzapetakis, M.F.,Nobrega, C.F.,Carreira, C.,Saraiva, I.H. (登録日: 2015-03-10, 公開日: 2016-01-20, 最終更新日: 2024-05-15)

主引用文献

Nobrega, C.S.,Saraiva, I.H.,Carreira, C.,Devreese, B.,Matzapetakis, M.,Pauleta, S.R.
The solution structure of the soluble form of the lipid-modified azurin from Neisseria gonorrhoeae, the electron donor of cytochrome c peroxidase.
Biochim.Biophys.Acta, 1857:169-176, 2016

PubMed Abstract: Neisseria gonorrhoeae colonizes the genitourinary track, and in these environments, especially in the female host, the bacteria are subjected to low levels of oxygen, and reactive oxygen and nitrosyl species. Here, the biochemical characterization of N. gonorrhoeae Laz is presented, as well as, the solution structure of its soluble domain determined by NMR. N. gonorrhoeae Laz is a type 1 copper protein of the azurin-family based on its spectroscopic properties and structure, with a redox potential of 277±5 mV, at pH7.0, that behaves as a monomer in solution. The globular Laz soluble domain adopts the Greek-key motif, with the copper center located at one end of the β-barrel coordinated by Gly48, His49, Cys113, His118 and Met122, in a distorted trigonal geometry. The edge of the His118 imidazole ring is water exposed, in a surface that is proposed to be involved in the interaction with its redox partners. The heterologously expressed Laz was shown to be a competent electron donor to N. gonorrhoeae cytochrome c peroxidase. This is an evidence for its involvement in the mechanism of protection against hydrogen peroxide generated by neighboring lactobacilli in the host environment.

PubMed: 26589091
DOI: 10.1016/j.bbabio.2015.11.006

実験手法

SOLUTION NMR