2N0J

Solution NMR Structure of the 27 nucleotide engineered neomycin sensing riboswitch RNA-ribostamycin complex

2N0J の概要

• エントリーDOI: 10.2210/pdb2n0j/pdb
• NMR情報: BMRB: 25526
• 分子名称: RNA_(27-MER), RIBOSTAMYCIN (2 entities in total)
• 機能のキーワード: riboswitches, rna-ligand interaction, aminoglycosides, rna recognition, rna structure, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9011.50

構造登録者

Duchardt-Ferner, E.,Gottstein-Schmidtke, S.R.,Weigand, J.E.,Ohlenschlaeger, O.E.,Wurm, J.,Hammann, C.,Suess, B.,Woehnert, J. (登録日: 2015-03-09, 公開日: 2016-02-03, 最終更新日: 2024-05-01)

主引用文献

Duchardt-Ferner, E.,Gottstein-Schmidtke, S.R.,Weigand, J.E.,Ohlenschlager, O.,Wurm, J.P.,Hammann, C.,Suess, B.,Wohnert, J.
What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.
Angew.Chem.Int.Ed.Engl., 55:1527-1530, 2016

PubMed Abstract: To ensure appropriate metabolic regulation, riboswitches must discriminate efficiently between their target ligands and chemically similar molecules that are also present in the cell. A remarkable example of efficient ligand discrimination is a synthetic neomycin-sensing riboswitch. Paromomycin, which differs from neomycin only by the substitution of a single amino group with a hydroxy group, also binds but does not flip the riboswitch. Interestingly, the solution structures of the two riboswitch-ligand complexes are virtually identical. In this work, we demonstrate that the local loss of key intermolecular interactions at the substitution site is translated through a defined network of intramolecular interactions into global changes in RNA conformational dynamics. The remarkable specificity of this riboswitch is thus based on structural dynamics rather than static structural differences. In this respect, the neomycin riboswitch is a model for many of its natural counterparts.

PubMed: 26661511
DOI: 10.1002/anie.201507365

実験手法

SOLUTION NMR