2MZ6

NMR structure of Protegrin-3 (PG3) in the presence of DPC micelles

Summary for 2MZ6

• Entry DOI: 10.2210/pdb2mz6/pdb
• Related: 2MZ5
• NMR Information: BMRB: 25474
• Descriptor: Protegrin-3 (1 entity in total)
• Functional Keywords: protegrin, antimicrobial protein, dpc micelle, dimer
• Biological source: Sus scrofa (pigs,swine,wild boar)
• Cellular location: Secreted: P32196
• Total number of polymer chains: 2
• Total formula weight: 4133.06

Authors

Usachev, K.S.,Efimov, S.V.,Kolosova, O.A.,Klochkova, E.A.,Aganov, A.V.,Klochkov, V.V. (deposition date: 2015-02-06, release date: 2015-03-25, Last modification date: 2024-10-30)

Primary citation

Usachev, K.S.,Efimov, S.V.,Kolosova, O.A.,Klochkova, E.A.,Aganov, A.V.,Klochkov, V.V.
Antimicrobial peptide protegrin-3 adopt an antiparallel dimer in the presence of DPC micelles: a high-resolution NMR study.
J.Biomol.Nmr, 62:71-79, 2015

PubMed Abstract: A tendency to dimerize in the presence of lipids was found for the protegrin. The dimer formation by the protegrin-1 (PG-1) is the first step for further oligomeric membrane pore formation. Generally there are two distinct model of PG-1 dimerization in either a parallel or antiparallel β-sheet. But despite the wealth of data available today, protegrin dimer structure and pore formation is still not completely understood. In order to investigate a more detailed dimerization process of PG-1 and if it will be the same for another type of protegrins, in this work we used a high-resolution NMR spectroscopy for structure determination of protegrin-3 (RGGGL-CYCRR-RFCVC-VGR) in the presence of perdeuterated DPC micelles and demonstrate that PG-3 forms an antiparallel NCCN dimer with a possible association of these dimers. This structural study complements previously published solution, solid state and computational studies of PG-1 in various environments and validate the potential of mean force simulations of PG-1 dimers and association of dimers to form octameric or decameric β-barrels.

PubMed: 25786621
DOI: 10.1007/s10858-015-9920-0

Experimental method

SOLUTION NMR