2MZ1

Solution structure of hnRNP C RRM in complex with 5'-UUUUC-3' RNA

2MZ1 の概要

• エントリーDOI: 10.2210/pdb2mz1/pdb
• NMR情報: BMRB: 25469
• 分子名称: Heterogeneous nuclear ribonucleoproteins C1/C2, 5'-R(*UP*UP*UP*UP*C)-3' (2 entities in total)
• 機能のキーワード: hnrnp c, rrm, complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P07910
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12776.81

構造登録者

Cienikova, Z.,Damberger, F.F.,Hall, J.,Allain, F.H.-T.,Maris, C. (登録日: 2015-02-05, 公開日: 2015-04-08, 最終更新日: 2024-05-01)

主引用文献

Cienikova, Z.,Damberger, F.F.,Hall, J.,Allain, F.H.,Maris, C.
Structural and mechanistic insights into poly(uridine) tract recognition by the hnRNP C RNA recognition motif.
J.Am.Chem.Soc., 136:14536-14544, 2014

PubMed Abstract: HnRNP C is a ubiquitous RNA regulatory factor and the principal constituent of the nuclear hnRNP core particle. The protein contains one amino-terminal RNA recognition motif (RRM) known to bind uridine (U)-rich sequences. This work provides a molecular and mechanistic understanding of this interaction. We solved the solution structures of the RRM in complex with poly(U) oligomers of five and seven nucleotides. The five binding pockets of RRM recognize uridines with an unusual 5'-to-3' gradient of base selectivity. The target recognition is therefore strongly sensitive to base clustering, explaining the preference for contiguous uridine tracts. Using a novel approach integrating the structurally derived recognition consensus of the RRM with a thermodynamic description of its multi-register binding, we modeled the saturation of cellular uridine tracts by this protein. The binding pattern is remarkably consistent with the experimentally observed transcriptome-wide cross-link distribution of the full-length hnRNP C on short uridine tracts. This result re-establishes the RRM as the primary RNA-binding domain of the hnRNP C tetramer and provides a proof of concept for interpreting high-throughput interaction data using structural approaches.

PubMed: 25216038
DOI: 10.1021/ja507690d

実験手法

SOLUTION NMR