2MY7

NMR Structure of unfolding intermediate state of RRM-3 domain of ETR-3

「2MIK」から置き換えられました

2MY7 の概要

• エントリーDOI: 10.2210/pdb2my7/pdb
• 関連するPDBエントリー: 2MY8
• NMR情報: BMRB: 19685
• 分子名称: CUGBP Elav-like family member 2 (1 entity in total)
• 機能のキーワード: etr 3 rrm-3, intermediate state, protein folding, rna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10930.56

構造登録者

Bhatt, H.P.,Ganguly, A.K.,Bhavesh, N.S. (登録日: 2015-01-21, 公開日: 2015-02-04, 最終更新日: 2024-05-15)

主引用文献

Bhatt, H.,Ganguly, A.K.,Sharma, S.,Kushwaha, G.S.,Firoz Khan, M.,Sen, S.,Bhavesh, N.S.
Structure of an Unfolding Intermediate of an RRM Domain of ETR-3 Reveals Its Native-like Fold.
Biophys.J., 118:352-365, 2020

PubMed Abstract: Prevalence of one or more partially folded intermediates during protein unfolding with different secondary and ternary conformations has been identified as an integral character of protein unfolding. These transition-state species need to be characterized structurally for elucidation of their folding pathways. We have determined the three-dimensional structure of an intermediate state with increased conformational space sampling under urea-denaturing condition. The protein unfolds completely at 10 M urea but retains residual secondary structural propensities with restricted motion. Here, we describe the native state, observable intermediate state, and unfolded state for ETR-3 RRM-3, which has canonical RRM fold. These observations can shed more light on unfolding events for RRM-containing proteins.

PubMed: 31866002
DOI: 10.1016/j.bpj.2019.11.3392

実験手法

SOLUTION NMR