2MY1

Solution structure of Bud31p

Summary for 2MY1

• Entry DOI: 10.2210/pdb2my1/pdb
• NMR Information: BMRB: 25439
• Descriptor: Pre-mRNA-splicing factor BUD31, ZINC ION (2 entities in total)
• Functional Keywords: zinc cluster, splicing protein, zinc finger, splicing
• Biological source: Saccharomyces cerevisiae S288c (Baker's yeast)
• Cellular location: Nucleus : P25337
• Total number of polymer chains: 1
• Total formula weight: 18750.72

Authors

van Roon, A.M.,Yang, J.,Mathieu, D.,Bermel, W.,Nagai, K.,Neuhaus, D. (deposition date: 2015-01-19, release date: 2015-03-11, Last modification date: 2024-05-15)

Primary citation

van Roon, A.M.,Yang, J.C.,Mathieu, D.,Bermel, W.,Nagai, K.,Neuhaus, D.
(113) Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
Angew.Chem.Int.Ed.Engl., 54:4861-4864, 2015

PubMed Abstract: Establishing the binding topology of structural zinc ions in proteins is an essential part of their structure determination by NMR spectroscopy. Using (113)Cd NMR experiments with (113)Cd-substituted samples is a useful approach but has previously been limited mainly to very small protein domains. Here we used (113)Cd NMR spectroscopy during structure determination of Bud31p, a 157-residue yeast protein containing an unusual Zn3Cys9 cluster, demonstrating that recent hardware developments make this approach feasible for significantly larger systems.

PubMed: 25703931
DOI: 10.1002/anie.201412210

Experimental method

SOLUTION NMR