2MY1

Solution structure of Bud31p

2MY1 の概要

• エントリーDOI: 10.2210/pdb2my1/pdb
• NMR情報: BMRB: 25439
• 分子名称: Pre-mRNA-splicing factor BUD31, ZINC ION (2 entities in total)
• 機能のキーワード: zinc cluster, splicing protein, zinc finger, splicing
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• 細胞内の位置: Nucleus : P25337
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18750.72

構造登録者

van Roon, A.M.,Yang, J.,Mathieu, D.,Bermel, W.,Nagai, K.,Neuhaus, D. (登録日: 2015-01-19, 公開日: 2015-03-11, 最終更新日: 2024-05-15)

主引用文献

van Roon, A.M.,Yang, J.C.,Mathieu, D.,Bermel, W.,Nagai, K.,Neuhaus, D.
(113) Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
Angew.Chem.Int.Ed.Engl., 54:4861-4864, 2015

PubMed Abstract: Establishing the binding topology of structural zinc ions in proteins is an essential part of their structure determination by NMR spectroscopy. Using (113)Cd NMR experiments with (113)Cd-substituted samples is a useful approach but has previously been limited mainly to very small protein domains. Here we used (113)Cd NMR spectroscopy during structure determination of Bud31p, a 157-residue yeast protein containing an unusual Zn3Cys9 cluster, demonstrating that recent hardware developments make this approach feasible for significantly larger systems.

PubMed: 25703931
DOI: 10.1002/anie.201412210

実験手法

SOLUTION NMR