2MWP

Solution structure of 53BP1 tandem Tudor domains in complex with a p53K382me2 peptide

2MWP の概要

• エントリーDOI: 10.2210/pdb2mwp/pdb
• 関連するPDBエントリー: 2MWO
• NMR情報: BMRB: 25348
• 分子名称: Tumor suppressor p53-binding protein 1, Cellular tumor antigen p53 (2 entities in total)
• 機能のキーワード: cell cycle, dna damage response, transcription-antitumor protein complex, transcription/antitumor protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q12888; Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15440.61

構造登録者

Cui, G.,Botuyan, M.V.,Mer, G. (登録日: 2014-11-15, 公開日: 2014-12-10, 最終更新日: 2025-03-26)

主引用文献

Tong, Q.,Cui, G.,Botuyan, M.V.,Rothbart, S.B.,Hayashi, R.,Musselman, C.A.,Singh, N.,Appella, E.,Strahl, B.D.,Mer, G.,Kutateladze, T.G.
Structural Plasticity of Methyllysine Recognition by the Tandem Tudor Domain of 53BP1.
Structure, 23:312-321, 2015

PubMed Abstract: p53 is dynamically regulated through various posttranslational modifications (PTMs), which differentially modulate its function and stability. The dimethylated marks p53K370me2 and p53K382me2 are associated with p53 activation or stabilization and both are recognized by the tandem Tudor domain (TTD) of 53BP1, a p53 cofactor. Here we detail the molecular mechanisms for the recognition of p53K370me2 and p53K382me2 by 53BP1. The solution structures of TTD in complex with the p53K370me2 and p53K382me2 peptides show a remarkable plasticity of 53BP1 in accommodating these diverse dimethyllysine-containing sequences. We demonstrate that dimeric TTDs are capable of interacting with the two PTMs on a single p53K370me2K382me2 peptide, greatly strengthening the 53BP1-p53 interaction. Analysis of binding affinities of TTD toward methylated p53 and histones reveals strong preference of 53BP1 for p53K382me2, H4K20me2, and H3K36me2 and suggests a possible role of multivalent contacts of 53BP1 in p53 targeting to and accumulation at the sites of DNA damage.

PubMed: 25579814
DOI: 10.1016/j.str.2014.11.013

実験手法

SOLUTION NMR