2MVO

Solution structure of the lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024

2MVO の概要

• エントリーDOI: 10.2210/pdb2mvo/pdb
• NMR情報: BMRB: 25277
• 分子名称: Putative lipoprotein (1 entity in total)
• 機能のキーワード: lipoprotein, lantibiotic resistance, lipid binding protein
• 由来する生物種: Microbispora sp. ATCC PTA-5024
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15456.09

構造登録者

Pozzi, R.,Schwartz, P.,Linke, D.,Kulik, A.,Nega, M.,Wohlleben, W.,Stegmann, E.,Coles, M. (登録日: 2014-10-09, 公開日: 2015-07-15, 最終更新日: 2024-11-06)

主引用文献

Pozzi, R.,Coles, M.,Linke, D.,Kulik, A.,Nega, M.,Wohlleben, W.,Stegmann, E.
Distinct mechanisms contribute to immunity in the lantibiotic NAI-107 producer strain Microbispora ATCC PTA-5024.
Environ Microbiol, 18:118-132, 2016

PubMed Abstract: The investigation of self-resistance in antibiotic producers is important to understand the emergence of antibiotic resistance in pathogens and to improve antibiotic production. Lantibiotics are ribosomally synthesized antibiotics that mostly target peptidoglycan biosynthesis. The actinomycete Microbispora ATCC PTA-5024 produces the lantibiotic NAI-107, which interferes with peptidoglycan biosynthesis by binding bactoprenol-pyrophosphate-coupled peptidoglycan precursors. In order to understand how Microbispora counteracts the action of its own antibiotic, its peptidoglycan composition was analysed in detail. Microbispora peptidoglycan consists of muropeptides with D-Ala and Gly in similar proportion at the fourth position of the peptide stems and alternative 3-3 cross-links besides the classical 4-3 cross-links. In addition, the NAI-107 biosynthetic gene cluster (mlb) was analysed for the expression of immunity proteins. We show that distinct immunity determinants are encoded in the mlb cluster: the ABC transporter MlbYZ acting cooperatively with the transmembrane protein MlbJ and the lipoprotein MlbQ. NMR structural analysis of MlbQ revealed a hydrophobic surface patch, which is proposed to bind the cognate lantibiotic. This study demonstrates that immunity in Microbispora is not only based on one determinant but on the action of the distinct immunity proteins MlbQ, MlbYZ and MlbJ.

PubMed: 25923468
DOI: 10.1111/1462-2920.12892

実験手法

SOLUTION NMR