2MTV

Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA

2MTV の概要

• エントリーDOI: 10.2210/pdb2mtv/pdb
• NMR情報: BMRB: 25188
• 分子名称: YTH domain-containing protein 1, RNA_(5'-R(*UP*GP*(6MZ)P*CP*AP*C)-3') (2 entities in total)
• 機能のキーワード: yth, m6a, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats); 詳細
• 細胞内の位置: Nucleus : Q9QY02
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19397.41

構造登録者

Theler, D.,Dominguez, C.,Blatter, M.,Boudet, J.,Allain, F.H.-T. (登録日: 2014-09-01, 公開日: 2014-11-26, 最終更新日: 2024-05-01)

主引用文献

Theler, D.,Dominguez, C.,Blatter, M.,Boudet, J.,Allain, F.H.
Solution structure of the YTH domain in complex with N6-methyladenosine RNA: a reader of methylated RNA.
Nucleic Acids Res., 42:13911-13919, 2014

PubMed Abstract: N(6)A methylation is the most abundant RNA modification occurring within messenger RNA. Impairment of methylase or demethylase functions are associated with severe phenotypes and diseases in several organisms. Beside writer and eraser enzymes of this dynamic RNA epigenetic modification, reader proteins that recognize this modification are involved in numerous cellular processes. Although the precise characterization of these reader proteins remains unknown, preliminary data showed that most potential reader proteins contained a conserved YT521-B homology (YTH) domain. Here we define the YTH domain of rat YT521-B as a N(6)-methylated adenosine reader domain and report its solution structure in complex with a N(6)-methylated RNA. The structure reveals a binding preference for NGANNN RNA hexamer and a deep hydrophobic cleft for m(6)A recognition. These findings establish a molecular function for YTH domains as m(6)A reader domains and should guide further studies into the biological functions of YTH-containing proteins in m(6)A recognition.

PubMed: 25389274
DOI: 10.1093/nar/gku1116

実験手法

SOLUTION NMR