2MTM

NMR structure of RCB-1 peptide

2MTM の概要

• エントリーDOI: 10.2210/pdb2mtm/pdb
• NMR情報: BMRB: 25169
• 分子名称: Putative uncharacterized protein (1 entity in total)
• 機能のキーワード: biomarker, natural product, unknown function
• 由来する生物種: Ricinus communis (Castor bean)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2073.63

構造登録者

Boldbaatar, D.,Elseedi, H.R. (登録日: 2014-08-21, 公開日: 2015-09-02, 最終更新日: 2024-11-06)

主引用文献

Boldbaatar, D.,Gunasekera, S.,El-Seedi, H.R.,Goransson, U.
Synthesis, Structural Characterization, and Bioactivity of the Stable Peptide RCB-1 from Ricinus communis.
J Nat Prod, 78:2545-2551, 2015

PubMed Abstract: The Ricinus communis biomarker peptides RCB-1 to -3 comprise homologous sequences of 19 (RCB-1) or 18 (RCB-2 and -3) amino acid residues. They all include four cysteine moieties, which form two disulfide bonds. However, neither the 3D structure nor the biological activity of any of these peptides is known. The synthesis of RCB-1, using microwave-assisted, Fmoc-based solid-phase peptide synthesis, and a method for its oxidative folding are reported. The tertiary structure of RCB-1, subsequently established using solution-state NMR, reveals a twisted loop fold with antiparallel β-sheets reinforced by the two disulfide bonds. Moreover, RCB-1 was tested for antibacterial, antifungal, and cytotoxic activity, as well as in a serum stability assay, in which it proved to be remarkably stable.

PubMed: 26509914
DOI: 10.1021/acs.jnatprod.5b00463

実験手法

SOLUTION NMR