2MSY

Solution structure of Hox homeodomain

2MSY の概要

• エントリーDOI: 10.2210/pdb2msy/pdb
• NMR情報: BMRB: 25142
• 分子名称: Homeobox protein Hox-C9 (1 entity in total)
• 機能のキーワード: homeodomain, transcription factor, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P31274
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8677.26

構造登録者

Kim, H.,Park, S.,Han, J.,Lee, B. (登録日: 2014-08-11, 公開日: 2015-09-16, 最終更新日: 2024-05-15)

主引用文献

Kim, H.H.,Park, S.J.,Han, J.H.,Pathak, C.,Cheong, H.K.,Lee, B.J.
Structural insight into the interaction between the Hox and HMGB1 and understanding of the HMGB1-enhancing effect of Hox-DNA binding.
Biochim.Biophys.Acta, 1854:449-459, 2015

PubMed Abstract: The Hox DNA binding domain, the homeodomain, plays critical roles in genetic control of development and cell fate determination. The variable regulatory functions of Hox proteins are accomplished by binding to target DNA sequences and collaborating protein partners that includes human high mobility group B1 (HMGB1). To better understand the interaction between Hox and HMGB1 and the facilitation of Hox-DNA binding by HMGB1, we solved the solution structure of the homeodomain of Hox including the N-terminal arm region (Hoxc9DBD hereafter). In addition, the details of the interaction between these two proteins, as well as DNA binding of the Hox-HMGB1 complex, were investigated by NMR, ITC, and EMSA. The results suggest that binding of the HMGB1 A-box to Hoxc9DBD makes the loop-1 (loop preceding helix-2 of Hoxc9DBD) more access to DNA backbone, which facilitate Hox-DNA binding with enhanced affinity.

PubMed: 25707357
DOI: 10.1016/j.bbapap.2015.02.009

実験手法

SOLUTION NMR