2MS1

Solution NMR structure of tRNApro:MLV Nucleocapsid Protein (1:1) Complex

2MS1 の概要

• エントリーDOI: 10.2210/pdb2ms1/pdb
• 関連するPDBエントリー: 2MQT 2MQV 2MS0
• NMR情報: BMRB: 25101
• 分子名称: Nucleocapsid protein p10, tRNApro, ZINC ION (3 entities in total)
• 機能のキーワード: rna/protein, viral protein-rna complex, retroviral primer annealing, nucleocapsid chaperone, viral protein/rna
• 由来する生物種: Murine leukemia virus; 詳細
• 細胞内の位置: Gag-Pol polyprotein: Host cell membrane ; Lipid-anchor . Matrix protein p15: Virion . Capsid protein p30: Virion . Nucleocapsid protein p10: Virion : P03355
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29387.22

構造登録者

D'Souza, V.,Yildiz, Z. (登録日: 2014-07-19, 公開日: 2014-09-10, 最終更新日: 2024-05-01)

主引用文献

Miller, S.B.,Yildiz, F.Z.,Lo, J.A.,Wang, B.,D'Souza, V.M.
A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing.
Nature, 515:591-595, 2014

PubMed Abstract: To prime reverse transcription, retroviruses require annealing of a transfer RNA molecule to the U5 primer binding site (U5-PBS) region of the viral genome. The residues essential for primer annealing are initially locked in intramolecular interactions; hence, annealing requires the chaperone activity of the retroviral nucleocapsid (NC) protein to facilitate structural rearrangements. Here we show that, unlike classical chaperones, the Moloney murine leukaemia virus NC uses a unique mechanism for remodelling: it specifically targets multiple structured regions in both the U5-PBS and tRNA(Pro) primer that otherwise sequester residues necessary for annealing. This high-specificity and high-affinity binding by NC consequently liberates these sequestered residues--which are exactly complementary--for intermolecular interactions. Furthermore, NC utilizes a step-wise, entropy-driven mechanism to trigger both residue-specific destabilization and residue-specific release. Our structures of NC bound to U5-PBS and tRNA(Pro) reveal the structure-based mechanism for retroviral primer annealing and provide insights as to how ATP-independent chaperones can target specific RNAs amidst the cellular milieu of non-target RNAs.

PubMed: 25209668
DOI: 10.1038/nature13709

実験手法

SOLUTION NMR