2MQW

Solution structure of a proteasome related subunit N terminal domain

2MQW の概要

• エントリーDOI: 10.2210/pdb2mqw/pdb
• 関連するPDBエントリー: 2MQX 2MR3
• NMR情報: BMRB: 19220
• 分子名称: 26S proteasome regulatory subunit RPN9 (1 entity in total)
• 機能のキーワード: a-helix bundle, hydrolase
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19206.71

構造登録者

Wu, Y.,Hu, Y.,Jin, C. (登録日: 2014-06-30, 公開日: 2015-02-04, 最終更新日: 2024-05-01)

主引用文献

Hu, Y.,Wu, Y.,Li, Q.,Zhang, W.,Jin, C.
Solution structure of yeast Rpn9: insights into proteasome lid assembly.
J. Biol. Chem., 290:6878-6889, 2015

PubMed Abstract: The regulatory particle (RP) of the 26 S proteasome functions in preparing polyubiquitinated substrates for degradation. The lid complex of the RP contains an Rpn8-Rpn11 heterodimer surrounded by a horseshoe-shaped scaffold formed by six proteasome-COP9/CSN-initiation factor (PCI)-containing subunits. The PCI domains are essential for lid assembly, whereas the detailed molecular mechanisms remain elusive. Recent cryo-EM studies at near-atomic resolution provided invaluable information on the RP architecture in different functional states. Nevertheless, atomic resolution structural information on the RP is still limited, and deeper understanding of RP assembly mechanism requires further studies on the structures and interactions of individual subunits or subcomplexes. Herein we report the high-resolution NMR structures of the PCI-containing subunit Rpn9 from Saccharomyces cerevisiae. The 45-kDa protein contains an all-helical N-terminal domain and a C-terminal PCI domain linked via a semiflexible hinge. The N-terminal domain mediates interaction with the ubiquitin receptor Rpn10, whereas the PCI domain mediates interaction with the neighboring PCI subunit Rpn5. The Rpn9-Rpn5 interface highlights two structural motifs on the winged helix module forming a hydrophobic center surrounded by ionic pairs, which is a common pattern for all PCI-PCI interactions in the lid. The results suggest that divergence in surface composition among different PCI pairs may contribute to the modulation of lid assembly.

PubMed: 25631053
DOI: 10.1074/jbc.M114.626762

実験手法

SOLUTION NMR