2MPE

Solution NMR structure for B. pseudomallei BPSL1050

Summary for 2MPE

• Entry DOI: 10.2210/pdb2mpe/pdb
• NMR Information: BMRB: 19042
• Descriptor: BPSL1050 (1 entity in total)
• Functional Keywords: melioidosis, unknown function
• Biological source: Burkholderia pseudomallei
• Total number of polymer chains: 1
• Total formula weight: 14261.01

Authors

Gaudesi, D.,Musco, G.,Quilici, G. (deposition date: 2014-05-15, release date: 2015-03-11, Last modification date: 2024-05-15)

Primary citation

Gaudesi, D.,Peri, C.,Quilici, G.,Gori, A.,Ferrer-Navarro, M.,Conchillo-Sole, O.,Thomas, R.,Nithichanon, A.,Lertmemongkolchai, G.,Titball, R.,Daura, X.,Colombo, G.,Musco, G.
Structure-Based Design of a B Cell Antigen from B. pseudomallei.
Acs Chem.Biol., 10:803-812, 2015

PubMed Abstract: Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis.

PubMed: 25495888
DOI: 10.1021/cb500831y

Experimental method

SOLUTION NMR