2MPE

Solution NMR structure for B. pseudomallei BPSL1050

2MPE の概要

• エントリーDOI: 10.2210/pdb2mpe/pdb
• NMR情報: BMRB: 19042
• 分子名称: BPSL1050 (1 entity in total)
• 機能のキーワード: melioidosis, unknown function
• 由来する生物種: Burkholderia pseudomallei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14261.01

構造登録者

Gaudesi, D.,Musco, G.,Quilici, G. (登録日: 2014-05-15, 公開日: 2015-03-11, 最終更新日: 2024-05-15)

主引用文献

Gaudesi, D.,Peri, C.,Quilici, G.,Gori, A.,Ferrer-Navarro, M.,Conchillo-Sole, O.,Thomas, R.,Nithichanon, A.,Lertmemongkolchai, G.,Titball, R.,Daura, X.,Colombo, G.,Musco, G.
Structure-Based Design of a B Cell Antigen from B. pseudomallei.
Acs Chem.Biol., 10:803-812, 2015

PubMed Abstract: Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis.

PubMed: 25495888
DOI: 10.1021/cb500831y

実験手法

SOLUTION NMR