2MP8

NMR structure of NKR-5-3B

2MP8 の概要

• エントリーDOI: 10.2210/pdb2mp8/pdb
• NMR情報: BMRB: 19970
• 分子名称: NKR-5-3B (1 entity in total)
• 機能のキーワード: bacteriocin, antimicrobial peptide, head-to-tail cyclic, helix bundle, antimicrobial protein
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6340.49

構造登録者

Rosengren, K.J.,Craik, D.J. (登録日: 2014-05-13, 公開日: 2015-05-13, 最終更新日: 2024-10-09)

主引用文献

Himeno, K.,Rosengren, K.J.,Inoue, T.,Perez, R.H.,Colgrave, M.L.,Lee, H.S.,Chan, L.Y.,Henriques, S.T.,Fujita, K.,Ishibashi, N.,Zendo, T.,Wilaipun, P.,Nakayama, J.,Leelawatcharamas, V.,Jikuya, H.,Craik, D.J.,Sonomoto, K.
Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium
Biochemistry, 54:4863-4876, 2015

PubMed Abstract: Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.

PubMed: 26174911
DOI: 10.1021/acs.biochem.5b00196

実験手法

SOLUTION NMR