2MP5

Structure of Bitistatin B

2MP5 の概要

• エントリーDOI: 10.2210/pdb2mp5/pdb
• 関連するPDBエントリー: 2mop
• NMR情報: BMRB: 19963
• 分子名称: Disintegrin bitistatin (1 entity in total)
• 機能のキーワード: bitis arietans, snake venom, disintegrin, toxin
• 由来する生物種: Bitis arietans (African puff adder)
• 細胞内の位置: Secreted: P17497
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9014.02

構造登録者

Carbajo, R.J.,Calvete, J.,Sanz, L.,Perez, A. (登録日: 2014-05-11, 公開日: 2014-11-12, 最終更新日: 2024-11-27)

主引用文献

Carbajo, R.J.,Sanz, L.,Perez, A.,Calvete, J.J.
NMR structure of bitistatin - a missing piece in the evolutionary pathway of snake venom disintegrins.
Febs J., 282:341-360, 2015

PubMed Abstract: Extant disintegrins, as found in the venoms of Viperidae and Crotalidae snakes (vipers and rattlesnakes, represent a family of polypeptides that block the function of β1 and β3 integrin receptors, both potently and with a high degree of selectivity. This toxin family owes its origin to the neofunctionalization of the extracellular region of an ADAM (a disintegrin and metalloprotease) molecule recruited into the snake venom gland proteome in the Jurassic. The evolutionary structural diversification of the disintegrin scaffold, from the ancestral long disintegrins to the more recently evolved medium-sized, dimeric and short disintegrins, involved the stepwise loss of pairs of class-specific disulfide linkages and the processing of the N-terminal region. NMR and crystal structures of medium-sized, dimeric and short disintegrins have been solved. However, the structure of a long disintegrin remained unknown. The present study reports the NMR solution structures of two disulfide bond conformers of the long disintegrin bitistatin from the African puff adder Bitis arietans. The findings provide insight into how a structural domain of the extracellular region of an ADAM molecule, recruited into and selectively expressed in the snake venom gland proteome as a PIII metalloprotease in the Jurassic, has subsequently been tranformed into a family of integrin receptor antagonists.

PubMed: 25363287
DOI: 10.1111/febs.13138

実験手法

SOLUTION NMR