2MNC

oligonucleotide model of miR-21 pre-element

2MNC の概要

• エントリーDOI: 10.2210/pdb2mnc/pdb
• NMR情報: BMRB: 19887
• 分子名称: RNA (5'-R(*GP*GP*GP*UP*UP*GP*AP*CP*CP*GP*UP*UP*GP*AP*AP*UP*CP*UP*CP*AP*CP*GP*GP*CP*AP*AP*CP*CP*C)-3') (1 entity in total)
• 機能のキーワード: mir-21, microrna, pre-element, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9275.56

構造登録者

Chirayil, S.,Wu, Q.,Amezcua, C.,Luebke, K. (登録日: 2014-04-02, 公開日: 2014-10-01, 最終更新日: 2024-05-01)

主引用文献

Chirayil, S.,Wu, Q.,Amezcua, C.,Luebke, K.J.
NMR Characterization of an Oligonucleotide Model of the MiR-21 Pre-Element.
Plos One, 9:e108231-e108231, 2014

PubMed Abstract: We have used NMR spectroscopy to characterize an oligonucleotide stem loop structure based on the pre-element of an oncogenic microRNA, miR-21. This predicted stem-loop structure is cleaved from the precursor of miR-21 (pre-miR-21) by the nuclease Dicer. It is also a critical feature recognized by the protein complex that converts the primary transcript (pri-miR-21) into the pre-miRNA. The secondary structure of the native sequence is poorly defined by NMR due to rapid exchange of imino protons with solvent; however, replacement of two adjacent putative G•U base pairs with G•C base pairs retains the conformation of the hairpin observed by chemical probing and stabilizes it sufficiently to observe most of the imino proton resonances of the molecule. The observed resonances are consistent with the predicted secondary structure. In addition, a peak due to a loop uridine suggests an interaction between it and a bulged uridine in the stem. Assignment of non-exchangeable proton resonances and characterization of NOEs and coupling constants allows inference of the following features of the structure: extrahelicity of a bulged adenosine, deviation from A-form geometry in a base-paired stem, and consecutive stacking of the adenosines in the 5' side of the loop, the guanosine of the closing base pair, and a cross-strand adenosine. Modeling of the structure by restrained molecular dynamics suggests a basis for the interaction between the loop uridine, the bulged uridine in the stem, and an A•U base pair in the stem.

PubMed: 25250627
DOI: 10.1371/journal.pone.0108231

実験手法

SOLUTION NMR