2MNA

The structural basis of DNA binding by the single-stranded DNA-binding protein from Sulfolobus solfataricus

2MNA の概要

• エントリーDOI: 10.2210/pdb2mna/pdb
• NMR情報: BMRB: 19095
• 分子名称: ssDNA, Single-stranded DNA binding protein (SSB) (2 entities in total)
• 機能のキーワード: dna repair, ssb, sulfolobus solfataricus, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14559.57

構造登録者

Gamsjaeger, R.,Kariawasam, R.,Gimenez, A.X.,Touma, C.F.,McIlwain, E.,Bernardo, R.E.,Shepherd, N.E.,Ataide, S.F.,Dong, A.Q.,Richard, D.J.,White, M.F.,Cubeddu, L. (登録日: 2014-04-02, 公開日: 2014-12-17, 最終更新日: 2024-05-15)

主引用文献

Gamsjaeger, R.,Kariawasam, R.,Gimenez, A.X.,Touma, C.,McIlwain, E.,Bernardo, R.E.,Shepherd, N.E.,Ataide, S.F.,Dong, Q.,Richard, D.J.,White, M.F.,Cubeddu, L.
The structural basis of DNA binding by the single-stranded DNA-binding protein from Sulfolobus solfataricus
Biochem.J., 465:337-346, 2015

PubMed Abstract: Canonical single-stranded DNA-binding proteins (SSBs) from the oligosaccharide/oligonucleotide-binding (OB) domain family are present in all known organisms and are critical for DNA replication, recombination and repair. The SSB from the hyperthermophilic crenarchaeote Sulfolobus solfataricus (SsoSSB) has a 'simple' domain organization consisting of a single DNA-binding OB fold coupled to a flexible C-terminal tail, in contrast with other SSBs in this family that incorporate up to four OB domains. Despite the large differences in the domain organization within the SSB family, the structure of the OB domain is remarkably similar all cellular life forms. However, there are significant differences in the molecular mechanism of ssDNA binding. We have determined the structure of the SsoSSB OB domain bound to ssDNA by NMR spectroscopy. We reveal that ssDNA recognition is modulated by base-stacking of three key aromatic residues, in contrast with the OB domains of human RPA and the recently discovered human homologue of SsoSSB, hSSB1. We also demonstrate that SsoSSB binds ssDNA with a footprint of five bases and with a defined binding polarity. These data elucidate the structural basis of DNA binding and shed light on the molecular mechanism by which these 'simple' SSBs interact with ssDNA.

PubMed: 25367669
DOI: 10.1042/BJ20141140

実験手法

SOLUTION NMR