2MN5

NMR structure of Copsin

2MN5 の概要

• エントリーDOI: 10.2210/pdb2mn5/pdb
• NMR情報: BMRB: 19880
• 分子名称: Copsin (1 entity in total)
• 機能のキーワード: antibiotic and antimicrobial protein, antimicrobial protein
• 由来する生物種: Coprinopsis cinerea
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6085.07

構造登録者

Hofmann, D.,Wider, G.,Essig, A.,Aebi, M. (登録日: 2014-03-28, 公開日: 2014-10-29, 最終更新日: 2024-11-06)

主引用文献

Essig, A.,Hofmann, D.,Munch, D.,Gayathri, S.,Kunzler, M.,Kallio, P.T.,Sahl, H.G.,Wider, G.,Schneider, T.,Aebi, M.
Copsin, a Novel Peptide-based Fungal Antibiotic Interfering with the Peptidoglycan Synthesis.
J.Biol.Chem., 289:34953-34964, 2014

PubMed Abstract: Fungi and bacteria compete with an arsenal of secreted molecules for their ecological niche. This repertoire represents a rich and inexhaustible source for antibiotics and fungicides. Antimicrobial peptides are an emerging class of fungal defense molecules that are promising candidates for pharmaceutical applications. Based on a co-cultivation system, we studied the interaction of the coprophilous basidiomycete Coprinopsis cinerea with different bacterial species and identified a novel defensin, copsin. The polypeptide was recombinantly produced in Pichia pastoris, and the three-dimensional structure was solved by NMR. The cysteine stabilized α/β-fold with a unique disulfide connectivity, and an N-terminal pyroglutamate rendered copsin extremely stable against high temperatures and protease digestion. Copsin was bactericidal against a diversity of Gram-positive bacteria, including human pathogens such as Enterococcus faecium and Listeria monocytogenes. Characterization of the antibacterial activity revealed that copsin bound specifically to the peptidoglycan precursor lipid II and therefore interfered with the cell wall biosynthesis. In particular, and unlike lantibiotics and other defensins, the third position of the lipid II pentapeptide is essential for effective copsin binding. The unique structural properties of copsin make it a possible scaffold for new antibiotics.

PubMed: 25342741
DOI: 10.1074/jbc.M114.599878

実験手法

SOLUTION NMR