2MMP

Solution structure of a ribosomal protein

2MMP の概要

• エントリーDOI: 10.2210/pdb2mmp/pdb
• NMR情報: BMRB: 19860
• 分子名称: Uncharacterized protein (1 entity in total)
• 機能のキーワード: ribosomal protein
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9223.80

構造登録者

Feng, Y. (登録日: 2014-03-17, 公開日: 2014-06-11, 最終更新日: 2024-05-15)

主引用文献

Feng, Y.,Song, X.,Lin, J.,Xuan, J.,Cui, Q.,Wang, J.
Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold.
Biochem.Biophys.Res.Commun., 450:67-72, 2014

PubMed Abstract: Three archaea-specific ribosomal proteins recently identified show no sequence homology with other known proteins. Here we determined the structure of L46a, the most conserved one among the three proteins, from Sulfolobus solfataricus P2 using NMR spectroscopy. The structure presents a twisted β-sheet formed by the N-terminal part and two helices at the C-terminus. The L46a structure has a positively charged surface which is conserved in the L46a protein family and is the potential rRNA-binding site. Searching homologous structures in Protein Data Bank revealed that the structure of L46a represents a novel protein fold. The backbone dynamics identified by NMR relaxation experiments reveal significant flexibility at the rRNA binding surface. The potential position of L46a on the ribosome was proposed by fitting the structure into a previous electron microscopy map of the ribosomal 50S subunit, which indicated that L46a contacts to domain I of 23S rRNA near a multifunctional ribosomal protein L7ae.

PubMed: 24875358
DOI: 10.1016/j.bbrc.2014.05.077

実験手法

SOLUTION NMR