2MLW

New Cyt-like delta-endotoxins from Dickeya dadantii - CytC protein

2MLW の概要

• エントリーDOI: 10.2210/pdb2mlw/pdb
• NMR情報: BMRB: 19834
• 分子名称: Type-1Ba cytolytic delta-endotoxin (1 entity in total)
• 機能のキーワード: cytolysin fold, modified greek key topology, toxin
• 由来する生物種: Dickeya dadantii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22412.26

構造登録者

Loth, K.,Costechareyre, D.,Effantin, G.,Rahbe, Y.,Condemine, G.,Landon, C.,Da Silva, P. (登録日: 2014-03-05, 公開日: 2015-02-18, 最終更新日: 2024-05-15)

主引用文献

Loth, K.,Costechareyre, D.,Effantin, G.,Rahbe, Y.,Condemine, G.,Landon, C.,da Silva, P.
New Cyt-like delta-endotoxins from Dickeya dadantii: structure and aphicidal activity.
Sci Rep, 5:8791-8791, 2015

PubMed Abstract: In the track of new biopesticides, four genes namely cytA, cytB, cytC and cytD encoding proteins homologous to Bacillus thuringiensis (Bt) Cyt toxins have been identified in the plant pathogenic bacteria Dickeya dadantii genome. Here we show that three Cyt-like δ-endotoxins from D. dadantii (CytA, CytB and CytC) are toxic to the pathogen of the pea aphid Acyrthosiphon pisum in terms of both mortality and growth rate. The phylogenetic analysis of the comprehensive set of Cyt toxins available in genomic databases shows that the whole family is of limited taxonomic occurrence, though in quite diverse microbial taxa. From a structure-function perspective the 3D structure of CytC and its backbone dynamics in solution have been determined by NMR. CytC adopts a cytolysin fold, structurally classified as a Cyt2-like protein. Moreover, the identification of a putative lipid binding pocket in CytC structure, which has been probably maintained in most members of the Cyt-toxin family, could support the importance of this lipid binding cavity for the mechanism of action of the whole family. This integrative approach provided significant insights into the evolutionary and functional history of D. dadantii Cyt toxins, which appears to be interesting leads for biopesticides.

PubMed: 25740111
DOI: 10.1038/srep08791

実験手法

SOLUTION NMR