2MKZ

solution structure of a protein C-terminal domain

2MKZ の概要

• エントリーDOI: 10.2210/pdb2mkz/pdb
• NMR情報: BMRB: 19801
• 分子名称: Proteasomal ubiquitin receptor ADRM1 (1 entity in total)
• 機能のキーワード: proteasome, uch37-binding domain, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q16186
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14822.52

構造登録者

Feng, Y.,Jiao, L. (登録日: 2014-02-17, 公開日: 2014-12-31, 最終更新日: 2024-05-15)

主引用文献

Jiao, L.,Ouyang, S.,Shaw, N.,Song, G.,Feng, Y.,Niu, F.,Qiu, W.,Zhu, H.,Hung, L.W.,Zuo, X.,Eleonora Shtykova, V.,Zhu, P.,Dong, Y.H.,Xu, R.,Liu, Z.J.
Mechanism of the Rpn13-induced activation of Uch37
Protein Cell, 5:616-630, 2014

PubMed Abstract: Uch37 is a de-ubiquitinating enzyme that is activated by Rpn13 and involved in the proteasomal degradation of proteins. The full-length Uch37 was shown to exhibit low iso-peptidase activity and is thought to be auto-inhibited. Structural comparisons revealed that within a homo-dimer of Uch37, each of the catalytic domains was blocking the other's ubiquitin (Ub)-binding site. This blockage likely prevented Ub from entering the active site of Uch37 and might form the basis of auto-inhibition. To understand the mode of auto-inhibition clearly and shed light on the activation mechanism of Uch37 by Rpn13, we investigated the Uch37-Rpn13 complex using a combination of mutagenesis, biochemical, NMR, and small-angle X-ray scattering (SAXS) techniques. Our results also proved that Uch37 oligomerized in solution and had very low activity against the fluorogenic substrate ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) of de-ubiquitinating enzymes. Uch37Δ(Hb,Hc,KEKE), a truncation removal of the C-terminal extension region (residues 256-329) converted oligomeric Uch37 into a monomeric form that exhibited iso-peptidase activity comparable to that of a truncation-containing the Uch37 catalytic domain only. We also demonstrated that Rpn13C (Rpn13 residues 270-407) could disrupt the oligomerization of Uch37 by sequestering Uch37 and forming a Uch37-Rpn13 complex. Uch37 was activated in such a complex, exhibiting 12-fold-higher activity than Uch37 alone. Time-resolved SAXS (TR-SAXS) and FRET experiments supported the proposed mode of auto-inhibition and the activation mechanism of Uch37 by Rpn13. Rpn13 activated Uch37 by forming a 1:1 stoichiometric complex in which the active site of Uch37 was accessible to Ub.

PubMed: 24752541
DOI: 10.1007/s13238-014-0046-z

実験手法

SOLUTION NMR